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citR [2017-8-11 16:0:3]
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citR [2017-8-11 16:0:3]

transcriptional repressor of citA
Locus
BSU09430
Isoelectric point
8.67
Molecular weight
35.44 kDa
Protein length
308 aa Sequence Blast
Gene length
924 bp Sequence Blast
Function
regulation of the minor citrate synthase
Product
transcriptional repressor (LysR family)
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,020,073 → 1,020,948

The protein

Protein family

Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
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    Biological materials

    Mutant

  • GP1283 (citA::aphA3), available in Jörg Stülke's lab
  • GP1753 (citR citA::aphA3), the resistance cassette can be cut out by introducing the cre-rekombinase into the chromosom of B. subtilis, available in Jörg Stülke's lab
  • GP2360 (citR citA::erm), the resistance cassette can be cut out by introducing the cre-rekombinase into the chromosom of B. subtilis, available in Jörg Stülke's lab
  • Expression vector

  • pGP2264 (N-terminal His-tag, purification from E. coli, in pETM-11), available in Jörg Stülke's lab
  • pGP1023 (C-terminal Strep-tag, purification from E. coli, in pGP574), available in Jörg Stülke's lab
  • pGP1029 (N-terminal Strep-tag, purification from B. subtilis, in pGP380), available in Jörg Stülke's lab
  • pGP1030 (C-terminal Strep-tag, purification from B. subtilis, in pGP382), available in Jörg Stülke's lab
  • pGP2260 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
  • pGP2262 (integration into ganA, expression under the control of the xylose-inducible PxylA promoter in B. subtilis, in pGP888), available in Jörg Stülke's lab
  • References

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